Search for: All records

Spider venom GDPD-like phospholipases D ( SicTox ) have been identified to be one of the major toxins in recluse spider venom. They are divided into two major clades: the α clade and the β clade. Most α clade toxins present high activity against lipids with choline head groups such as sphingomyelin, while activities in β clade toxins vary and include preference for substrates containing ethanolamine headgroups ( Sicarius terrosus , St_βIB1). A structural comparison of available structures of phospholipases D (PLDs) reveals a conserved aromatic cage in the α clade. To test the potential influence of the aromatic cage on membrane-lipid specificity we performed molecular dynamics (MD) simulations of the binding of several PLDs onto lipid bilayers containing choline headgroups; two SicTox from the α clade, Loxosceles intermedia αIA1 (Li_αIA) and Loxosceles laeta αIII1 (Ll_αIII1), and one from the β clade, St_βIB1. The simulation results reveal that the aromatic cage captures a choline-headgroup and suggest that the cage plays a major role in lipid specificity. We also simulated an engineered St_βIB1, where we introduced the aromatic cage, and this led to binding with choline-containing lipids. Moreover, a multiple sequence alignment revealed the conservation of the aromatic cage among the α clade PLDs. Here, we confirmed that the i-face of α and β clade PLDs is involved in their binding to choline and ethanolamine-containing bilayers, respectively. Furthermore, our results suggest a major role in choline lipid recognition of the aromatic cage of the α clade PLDs. The MD simulation results are supported by in vitro liposome binding assay experiments. 

Abstract We present optical and near-infrared (NIR) observations of SN 2022crv, a stripped-envelope supernova in NGC 3054, discovered within 12 hr of explosion by the Distance Less Than 40 Mpc Survey. We suggest that SN 2022crv is a transitional object on the continuum between Type Ib supernovae (SNe Ib) and Type IIb supernovae (SNe IIb). A high-velocity hydrogen feature (∼ −20,000 to −16,000 km s⁻¹) was conspicuous in SN 2022crv at early phases, and then quickly disappeared. We find that a hydrogen envelope of ∼10⁻³M_⊙can reproduce the observed behavior of the hydrogen feature. The lack of early envelope cooling emission implies that SN 2022crv had a compact progenitor with an extremely low amount of hydrogen. A nebular spectral analysis shows that SN 2022crv is consistent with the explosion of a He star with a final mass of ∼4.5–5.6M_⊙that evolved from a ∼16 to 22M_⊙zero-age main-sequence star in a binary system with ∼1.0–1.7M_⊙of oxygen finally synthesized in the core. In order to retain such a small amount of hydrogen, the initial orbital separation of the binary system is likely larger than ∼1000R_⊙. The NIR spectra of SN 2022crv show a unique absorption feature on the blue side of the Heiline at ∼1.005μm. This is the first time such a feature has been observed in SNe Ib/IIb, and it could be due to Sr II. Further detailed modeling of SN 2022crv can shed light on the progenitor and the origin of the mysterious absorption feature in the NIR.